ANTIBACTERIAL ACTIVITY OF ZINGIBER OFFICINALE (GINGER) AGAINST SELECTED DRUG RESISTANT ORGANISMS (Staphylococcus aureus and Escherichia coli)
Abstract
The aim of this research was to determine the antibacterial effects of the aqueous and ethanol extracts of Zingiber officinale (Ginger) against selected drug resistant organisms of Staphylococcus spp and Escherichia coli. We carried out antibacterial tests on the selected drug resistant organisms using paper disc diffusion by kirby-bauer disc diffusion method. Whiteman filter paper was cut into pieces of 9mm, sterilized with an autoclave and dipped into the different concentrations of the extracts that were serially diluted for 30minutes and placed on the pre-inoculated plates of the test organisms, incubated for 24hours after which zone of inhibition diameter (ZID) were measured in MM. The results indicated that 50mg/ml concentration and the control antibiotics(AUG) were effective against staphylococcus spp in the ethanol extract with ZID of 10 and 20.while 400mg/ml and control drug were effective against Escherichia coli for ethanol ginger extract with ZID of 11 and 10MM respectively. In aqueous ginger extract, 400mg/ml, 50mg/ml and control antibiotics showed zones of inhibition of 13, 10 and 11 respectively; 400mg/ml and control drug (AUG-30Mg) were 10 and 13 against E.coli. The MIC was 50mg/ml for Staphylococcus aureus on both aqueous and ethanol extracts while 400mg/ml is both the MIC and MBC for E. coli on both aqueous and ethanol extracts. Both the control and different concentrations of the extracts were active against the selected resistant organisms while some strains were still resistant to the extracts. The result showed that Ginger could be very effective as an antibacterial agent against drug resistant organisms of Staphylococcus aureus and Escherichia coli mostly at a high concentration dosages and dependent on the type of drug resistant organism.
Key words: Zingiber officinale, extracts, resistance, Staphylococcus aureus, Escherichia coli
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Ali B.H, Blunden G, Tanira M.O, Nemmar A. (2008). Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): a review of recent research. Food Chem Toxicol. 46:409–420.
Al-Tahtawy R.H.M, El-Bastawesy A.M, Abdel Monem M.G, Zekry Z.K, Al-Mehdar H.A, El-Merzabani M.M. (2011). Antioxidant activity of the volatile oils of Zingiber officinale (ginger) Spatula DD. 1:1–8.
Atai Z, Atapour M, Mohseni M. (2009). Inhibitory effect of Ginger Extract on Candida Albicans. Am J Applied Sci. 6:1067–1069.
Beceiro A, Tomás M, Bou G. (2013). Antimicrobial resistance and virulence: a successful or deleterious association in the bacterial world? Clin Microbiol Rev. 26:185–230.
Bellik Y. (2014). Total antioxidant activity and antimicrobial potency of the essential oil and oleoresin of Zingiber officinale Roscoe. Asian Pac J Trop Dis. 4:40–44.
Bevan E.R, Jones A.M, Hawkey P.M. (2017). Global epidemiology of CTX-M β-lactamases: temporal and geographical shifts in genotype. J Antimicrob Chemoth. 72:2145–2155.
Bhattarai S, Tran V.H, Duke C.C. (2001). The stability of gingerol and shogaol in aqueous solutions. J Pharm Sci. 90:1658–1664.
Blair J.M, Richmond G.E, Piddock L.J. (2014). Multidrug efflux pumps in Gram-negative bacteria and their role in antibiotic resistance. Future Microbiol. 9:1165–1177.
Chancey S.T, Zähner D, Stephens D.S. (2012). Acquired inducible antimicrobial resistance in Gram-positive bacteria. Future Microbiol. 7:959–978.
Choi Y.Y, Kim M.H, Hong J, Kim S, Yang W.M. (2013). Dried Ginger (Zingiber officinalis) Inhibits Inflammation in a Lipopolysaccharide-Induced Mouse Model. Evid Based Complement Alternat Med. 914563.
Coculescu B.I. (2009). Antimicrobial resistance induced by genetic changes. J Med Life. 2:114–123.
Costa S.S, Viveiros M, Amaral L. (2013). Multidrug efflux pumps in Staphylococcus aureus: an update. Open Microbiol J. 7:59–71.
Emmanuel, S.E., Ehinmitan, E.O., Bodunde, R.S and Joseph, J.C.(2021). Antimicrobial Activity of Zingiber Officinale and Alium sativum on some Drug resistant bacterial isolates. J.appl.Sci. Environ.Manage.25:6, 1053-1058.
Ficker C, Smith M.L, Akpagana K. (2003). Bioassay-guided isolation and identification of antifungal compounds from ginger. J Phytother Res. 17:897–903.
Filice G.A, Nyman J.A, Lexau C. (2010). Excess costs and utilization associated with methicillin resistance for patients with Staphylococcus aureus infection. Infect Cont Hosp Ep. 31:365–373.
Friedman N.D, Tomkin E, Carmeli Y. (2016). The negative impact of antibiotic resistance. Clin Microbiol Infect. 22:416–422.
Goossens H. (2009). Antibiotic consumption and link to resistance. Clin Microbiol Infec. 15 3:12–15.
Griffith M, Postelnick M, Scheetz M. (2012). Antimicrobial stewardship programs: methods of operation and suggested outcomes. Expert Rev Anti-Infe. 10:63–73.
Gull,I., Saeed,M.,Shaukat,H.,Anslam,S.M.,Samra, Z. Q and Atha, A. M(2012).Inhibitory effect of Allium sativum and Zingiber officinale extracts on clinically important drug resistant pathogenic bacteria. Annals of Clinically Microbio.Antimicrobials,11:8:1-6.
Habib S.H, Makpol S, Abdul Hamid N.A, Das S, Ngah W.Z, Yusof Y.A. (2008). Ginger Extract (Zingiber Officinale) has Anti-Cancer and Anti-Inflammatory Effects on Ethionine-Induced Hepatoma Rats. Clinics. 63:807–813.
Haghighi A, Tavalaei N, Owlia M.B. (2006). Effects of ginger on primary knee osteoarthritis. Indian J Rheumatol. 1:3–7.
Karna P, Chagani S, Gundala S.R, Rida P.C, Asif G, Sharma V, Gupta M.V, Aneja R. (2012). Benefits of whole ginger extract in prostate cancer. Br J Nutr. 107:473–84.
Kourtesi C, Ball A.R, Huang Y.Y. (2013). Microbial efflux systems and inhibitors: approaches to drug discovery and the challenge of clinical implementation. Open Microbiol J. 7:34–52.
Landers T.F, Cohen B, Wittum T.E. (2012). A review of antibiotic use in food animals: perspective, policy, and potential. Public Health Rep. 127:4–22.
Li Y, Tran V.H, Duke C.C, Roufogalis B.D. (2012). Preventive and Protective Properties of Zingi- ber officinale (Ginger) in Diabetes Mellitus, Diabetic Complications, and Associated Lipid and Other Metabolic Disorders: A Brief Review. Evid Based Complement Alternat Med:516870.
Ling H, Yang H, Tan S.H, Chui W.K, Chew E.H. (2010) 6-Shogaol, an active constituent of ginger, inhibits breast cancer cell invasion by reducing matrix metalloproteinase-9 expression via blockade of nuclear factor-κB activation. Br J Pharm. 161:1763–77.
Liu Y, Whelan R.J, Pattnaik B.R, Ludwig K, Subudhi E, Rowland H, Claussen N, Zucker N, Uppal S, Kushner D.M, Felder M, Patankar M.S, Kapur A. (2012). Terpenoids from Zingiber officinale (Ginger) induce apoptosis in endometrial cancer cells through the activation of p53. PLoS One. 7:e53178.
Mah T.F. (2012). Biofilm-specific antibiotic resistance. Future Microbiol. 7:1061–1072.
Maiti R, Jana D, Das U.K, Ghosh D. (2004). Antidiabetic effect of aqueous extract of seed of Tamarindus indica in streptozotocin-induced diabetic rats. J Ethnopharmacol. 92:85–91.
Maragakis L.L, Perencevich E.N, Cosgrove S.E. (2008). Clinical and economic burden of antimicrobial resistance. Expert Rev Anti-Infe. 6:751–763.
Martinez J.L. (2014). General principles of antibiotic resistance in bacteria. Drug Discov Today. 11:33–39.
Miller W.R, Munita J.M, Arias C.A. (2014). Mechanisms of antibiotic resistance in enterococci. Expert Rev Anti-Infe. 12:1221–1236.
Pakyz A, Powell J.P, Harpe S.E. (2008). Diversity of antimicrobial use and resistance in 42 hospitals in the United States. Pharmacotherapy. 28:906–912.
Putman M, van Veen H.W, Konings W.N. (2000). Molecular properties of bacterial multidrug transporters. Microbiol Mol Biol Rev. 64:672–693.
Rahmani A.H, Albutti A.S, Aly S.M. (2014). Therapeutics role of olive fruits/oil in the prevention of diseases via modulation of anti-oxidant, anti-tumour and genetic activity. Int J Clin Exp Med. 7:799–808.
Redgrave L.S, Sutton S.B, Webber M.A. (2014).Fluoroquinolone resistance: mechanisms, impact on bacteria, and role in evolutionary success. Trends Microbiol. 22:438–445.
Rong X, Peng G, Suzuki T, Yang Q, Yamahara J, Li Y. (2009). A 35-day gavage safety assessment of ginger in rats. Regul Toxicol Pharmacol. 54:118–23.
Saraswat M, Reddy P.Y, Muthenna P, Reddy G.B. (2009). Prevention of non-enzymic glycation of proteins by dietary agents: prospects for alleviating diabetic complications. Brit J Nutr. 1011714–1721.
Shyur L.F, Tsung J.H, Chen J.H, Chiu C.Y, Lo C.P. (2005). Antioxidant Properties of Extracts from Medicinal Plants Popularly Used in Taiwan Oxidatative stress play a siginificant effect in the pathogenesis of various types of disease. Int J Appl Sci Eng. 3:195–202.
Tacconelli E. (2009). Antimicrobial use: risk driver of multidrug resistant microorganisms in healthcare settings. Curr Opin Infect Dis. 22:352–358.
Tjendraputra E, Tran V.H, Biu-Brennan D, Roufogalis B.D, Duke C.C. (2001). Effect of ginger constituents and synthetic analogues on cyclooxygenase-2 enzyme in intact cells. Bioorg Chem. 29:156–163.
Toth M, Antunes N.T, Stewart N.K. (2016). Class D β-lactamases do exist in Gram-positive bacteria. Nat Chem Biol. 12:9–14.
Wegener H.C. (2012). Improving food safety through a One Health approach. Washington: National Academy of Sciences. Antibiotic resistance—linking human and animal health; pp. 331–349.
Wood T.K, Knabel S.J, Kwan B.W. (2013). Bacterial persister cell formation and dormancy. Appl Environ Microbiol. 79:7116–7121.
World Health Organization (2015). Global action plan on antimicrobial resistance. (2015).
World Health Organization (2014). World Health Statistics
Yu V.L. (2011) Guidelines for hospital-acquired pneumonia and health-care-associated pneumonia: a vulnerability, a pitfall, and a fatal flaw. Lancet Infect Dis. 11:248–252.
Zhanel G.G., Lawson C.D., Adam H. (2013) Ceftazidime-Avibactam: a novel cephalosporin/β-lactamase inhibitor combination. Drugs. 73:159–177.
Zick, S.M., Djuric, Z., Ruffin M.T, Litzinger A.J., Normolle D.P., Alrawi S, Feng M.R, Brenner D.E. (2008).Pharmacokinetics of 6-gingerol, 8-gingerol, 10-gingerol, and 6-shogaol and conjugate metabolites in healthy human subjects. Cancer Epidemiol Biomarkers Prev. 17:1930–1936.
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